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ESM Figure 1: Partial sequence alignment of CGMM101 marker (Primer ID TC77707) genic region across 20 chickpea accessions, showing bi-allelic and tri-allelic SNPs. The asterisks represent similar sequences.

ESM Figure 2: A schematic representation of the strategy for the development of CISR markers. An example showing alignment of a chickpea unigene to a gene on one Medicagotruncatula chromosome. The chickpea unigene shows similarity with two exons (Exon 1 and Exon 2) separated by one intron (Intron 1). To develop the CISR marker, primer pairs (F: forward primer, R: reverse primer) have been designed by using Exon 1 and Exon 2 sequences in such a way that they amplify the intronic region.

ESM Table 1: Details on primer pairs designed for homologous (chickpea) as well as heterologous (related legume) species. This ESM table provides the primer sequence information for 688 chickpea and 657 heterologous species derived genes.

ESM Table 2: Details on sequence diversity estimates of 220 genic regions in chickpea germplasm. This ESM table provides detailed sequence diversity of 220 genic regions in chickpea germplasm.

ESM Table 3: Details on identification of CAPS candidates. This ESM table provides the details for primer sequence information and restriction enzyme information of 279 CAPS candidates.

ESM Table 4: Polymorphism assessment of validated CAPS markers. This ESM table provides the details on polymorphism status of 143 CAPS validated CGMMs on one inter-specific and two intra-specific mapping populations.

ESM Table 5: Details on development and polymorphism assessment of CISR markers. This ESM table provides the details on primer sequence information and polymorphic status of 121 CISR markers on one inter-specific and two intra-specific mapping populations.

ESM Table 6: Details on polymorphism assessment of ICCeM markers. This ESM table provides the details on primer sequence information and polymorphic status of 77 EST-SSRs on one inter-specific and two intra-specific mapping populations.

Please contact : Dr Rajeev Varshney, Director-Center of Excellence in Genomics, ICRISAT, Patancheru, 502 324, India;
Office: +91 40 3071 3305; Email : r.k.varshney@cgiar.org
© Center of Excellence in Genomics. 2008